[Offer PDF] ISO 22196-2011 Measurement of antibacterial activity on plastics and

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[Offer PDF]  - 22 页数         ISO 22196
        Active         2011.08.01         Measurement of antibacterial activity on plastics and other non-porous surfaces - Second Edition
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Contents Page
Foreword ...........................................................................................................................................................iv
Introduction........................................................................................................................................................v
1 Scope.....................................................................................................................................................1
2 Normative references...........................................................................................................................1
3 Terms and definitions ...........................................................................................................................2
4 Materials ................................................................................................................................................2
4.1 Bacteria to be used for the tests..........................................................................................................2
4.2 Reagents, culture media and solutions ..............................................................................................3
5 Apparatus..............................................................................................................................................4
6 Sterilization of apparatus and storage of stock cultures ..................................................................5
6.1 Dry-heat sterilization .............................................................................................................................5
6.2 High-pressure steam sterilization........................................................................................................5
6.3 Preparation of glassware......................................................................................................................5
6.4 Maintenance of stock cultures.............................................................................................................5
7 Procedure..............................................................................................................................................6
7.1 Pre-culture of bacteria ..........................................................................................................................6
7.2 Preparation of test specimens .............................................................................................................6
7.3 Preparation of test inoculum................................................................................................................6
7.4 Inoculation of test specimens..............................................................................................................6
7.5 Incubation of the inoculated test specimens .....................................................................................8
7.6 Recovery of bacteria from test specimens.........................................................................................8
7.7 Determining the viable bacteria count by the pour plate culture method.......................................8
8 Expression of results...........................................................................................................................9
8.1 Determination of the number of viable bacteria.................................................................................9
8.2 Conditions for a valid test ....................................................................................................................9
8.3 Calculation of the antibacterial activity...............................................................................................9
8.4 Effectiveness of the antibacterial agent............................................................................................10
9 Repeatability and reproducibility.......................................................................................................10
10 Test report...........................................................................................................................................10
Annex A (normative) Quality of biological materials...................................................................................11
A.1 General ................................................................................................................................................11
A.2 Chemical composition of 1/500 nutrient broth (1/500 NB) ..............................................................11
Annex B (informative) Repeatability and reproducibility............................................................................12
B.1 Background.........................................................................................................................................12
B.2 Summary .............................................................................................................................................12
B.3 Experiment ..........................................................................................................................................12
B.4 Results and discussion ......................................................................................................................13
Bibliography.....................................................................................................................................................15

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